陈凯,江苏常州人,理学博士,副教授/硕导。
研究领域:环境微生物学,微生物生理与遗传学;
Email: chenkai@njau.edu.cn
地址:南京市玄武区卫岗1号 必赢626net入口首页生命科学楼C6011
Ø 承担课程:
² 本科生:《基础微生物学》、《基础微生物学实验》
² 研究生:《现代微生物学实验技术》
Ø 研究方向:
² 有机污染物的微生物降解与环境修复;
² 卤代芳烃的微生物脱卤机制;
² 代谢型质粒与宿主的适应性进化;
Ø 科研项目:
² 国家自然科学基金面上项目,32370126,遗传生物强化中广宿主代谢质粒pDCA-1的流行规律与高效修复机制,2024/01-2027/12,主持;
² 国家自然科学基金面上项目,32070091,菌株Pigmentiphaga sp. H8中4-羟基苯甲酸羟化酶基因冗余赋予宿主的选择性优势,2021/01-2024/12,主持;
² 国家重点研发计划,2022YFA0912501,水环境污染物降解元件挖掘和代谢通路解析,2022/11-2027/10,子课题主持;
² 国家重点研发计划,2018YFA0901202,高效降解通路的设计构建与底盘适配,2019/07-2024/06,子课题主持;
² 国家自然科学基金面上项目,31870095,卤代芳烃代谢质粒pBHB与宿主Comamonas sp. 7D-2的补偿性进化机制,2019/01-2022/12,主持;
² 中央高校基本科研业务费自主创新重点研究项目,KYZ201742, Pigmentiphaga sp. H8中3,5-二溴-4-羟基苯甲酸的代谢调控机制, 2017/01-2019/12,主持;
² 国家自然科学基金青年基金项目,31400105,菌株Pigmentiphaga sp. H8对3,5-二溴-4-羟基苯甲酸的降解及脱溴机制,2015/01-2017/12,主持;
² 中央高校基本科研业务费青年项目,KJQN201529,2015/01-2016/12,主持。
² 中国博士后科学基金特别资助,2015T80562,CnpR介导2,6-二氯-4-硝基苯酚降解的调控机制,2015-2017,主持;
² 中国博士后科学基金面上项目,2014M561666,菌株Ensifer sp. 22-1对2,6-二氯-4-硝基苯酚的降解机制,2014-2016,主持.
Ø 十篇代表论文
1. Long Zhang, Man Song, Zhenbo Mao, Yuan Liu, Feng Li, Jiandong Jiang*, Kai Chen*. A new enantioselective dioxygenase for the (S)-enantiomer of the chiral herbicide dichlorprop in Sphingopyxis sp. DBS4. International Biodeterioration & Biodegradation, 2023, 176, 105511.
2. Kai Chen, Xihui Xu, Muji Yang, Tairong Liu, Bin Liu, Jianchun Zhu, Baozhan Wang, Jiandong Jiang*. Genetic redundancy of 4-hydroxybenzoate 3-hydroxylase genes ensures the catabolic safety of Pigmentiphaga sp. H8 in 3-bromo-4-hydroxybenzoate-contaminated habitats. Environmental Microbiology, 2022, 24: 5123-5138.
3. Kai Chen, Zhuang Ke, Sicheng Wang, Shen Wang, Ke Yang, Weibin Jia, Jianchun Zhu, Jiandong Jiang*. Precise regulation of differential transcriptions of various catabolic genes by OdcR via a single nucleotide mutation in the promoter ensures the safety of metabolic flux. Applied and Environmental Microbiology, 2022, 88(18): e01182-22.
4. Zhuang Ke, Shen Wang, Wenqi Zhu, Fu Zhang, Wenjing Qiao*, Jiandong Jiang, Kai Chen *. Genetic bioaugmentation with triclocarban-catabolic plasmid effectively removes triclocarban from wastewater. Environmental Research, 2022, 113921.
5. Zhuang Ke, Shen Wang, Weixian Dai, Weibin Jia, Yang Mu, Jiandong Jiang *, Kai Chen *. Engineering of the chloroaniline-catabolic plasmid pDCA-1 and its potential for genetic bioaugmentation. International Biodeterioration & Biodegradation, 2022, 172:105435.
6. Zhuang Ke, Minjian Lan, Tunan Yang, Weibin Jia, Zhenjiu Gou, Kai Chen *, Jiandong Jiang *. A two-component monooxygenase for continuous denitration and dechlorination of chlorinated 4-nitrophenol in Ensifer sp. strain 22-1. Environmental Research, 2021, 198: 111216.
7. Kai Chen, Yang Mu, Shanshan Jian, Xiaoxia Zang, Qing Chen, Weibin Jia, Zhuang Ke, Yanzheng Gao, Jiandong Jiang*. Comparative transcriptome analysis reveals the mechanism underlying 3,5-dibromo-4-hydroxybenzoate catabolism via a new oxidative-decarboxylation pathway. Applied and Environmental Microbiology, 2018, 84(6): e02467-17.
8. Kai Chen#, Xihui Xu#, Long Zhang#, Zhenjiu Gou, Shunpeng Li, Shiri Freilich*, Jiandong Jiang*. Comparison of four comamonas catabolic plasmids reveals the evolution of pBHB to catabolize haloaromatics. Applied and Environmental Microbiology, 2016, 82(5): 1401-1411.
9. Kai Chen, Linglong Huang, Changfeng Xu, Xiaomei Liu, Jian He, Stephen H. Zinder, ShunPeng Li, Jiandong Jiang*. Molecular characterization of the enzymes involved in the degradation of a brominated aromatic herbicide. Molecular Microbiology, 2013, 89: 1121-1139.
10. Kai Chen, Yuan Liu, Dongmei Mao, Xiaomei Liu, Shunpeng Li, Jiandong Jiang*. An Essential Esterase (BroH) for the Mineralization of Bromoxynil Octanoate by a Natural Consortium of Sphingopyxis sp. Strain OB-3 and Comamonas sp. Strain 7D-2. Journal of Agricultural and Food Chemistry, 2013, 61(47): 11550-11559.
Ø 授权专利
1. 国家发明专利: 陈凯, 蒋建东, 李顺鹏, 苟镇久; 一种氯代硝基苯酚类化合物氧化还原酶基因簇cnpAB及其应用; 专利号: ZL201710722156.4; 申请日:2017-8-22; 授权公告日: 2020-6-26.
2. 国家发明专利: 蒋建东, 陈凯, 李顺鹏, 沐阳; 一种卤代对羟基苯甲酸氧化脱羧酶基因odcA及其应用; 专利号: ZL201710748337.4; 申请日:2017-8-25; 授权公告日: 2020-6-9.
3. 国家发明专利: 蒋建东, 陈凯; 3-溴-4-羟基苯甲酸氧化酶基因簇phbh2pcaA2B2及其应用; 专利号: CN202111331740.X; 申请日:2021-11-11; 授权公告日: 2023-05-26。